Phenylpropanoids and flavonoids from Phlomis kurdica as inhibitors of human lactate dehydrogenase
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چکیده
منابع مشابه
Human Lactate Dehydrogenase A Inhibitors: A Molecular Dynamics Investigation
Lactate dehydrogenase A (LDHA) is an important enzyme in fermentative glycolysis, generating most energy for cancer cells that rely on anaerobic respiration even under normal oxygen concentrations. This renders LDHA a promising molecular target for the treatment of various cancers. Several efforts have been made recently to develop LDHA inhibitors with nanomolar inhibition and cellular activity...
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The present study was designed to investigate the anti-melanogenic and cytotoxic activities of methanol extract of Phlomis kurdica. The antioxidant and anti-tyrosinase activity of MeOH extract from P. kurdica (MPk) were examined by DPPH radical scavenging and mushroom tyrosinase activity assays (in vitro), respectively. Furthermore, the effect of MPk on the melanin content, cellular tyrosinase ...
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Optimum reaction conditions at 30#{176} ± 0.5#{176} for two continuous spectrophotometric assay procedures, lactate to pyruvate (LP) and pyruvate to lactate (PL), were determined with respect to pH at 30#{176} (pH30) substrate concentration, and coenzyme concentration for the human LDH isoenxymes. For the P L procedure, broad pH30 optima were within the range of 7.20-7.40 for all the LDH isoenz...
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The presence of a new lactate dehydrogenase inhibitor on the trailing edge of the NADH peak from chromatography on diethylaminoethyl-celluose [Loshon et al., Clin. Chem., this issue] was verified. It was resolved from the NADH by high-performance liquid chromatography on muBondapak C18. When the new inhibitor was present in a reaction mixture to the extent that, of the initial 260-nm absorbance...
متن کاملFormation and properties of lactate dehydrogenase inhibitors in NADH.
We describe some characteristics of the mode of formation of inhibitors of lactate dehydrogenase from commercial NADH. Inhibitor formation is time- and concentration-dependent and also varies with the commercial source of NADH. At least two inhibitory components can form in concentrated NADH solutions. One of these can be separated from NADH by chromatography on either diethylaminoethyl-celluos...
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ژورنال
عنوان ژورنال: Phytochemistry
سال: 2015
ISSN: 0031-9422
DOI: 10.1016/j.phytochem.2015.03.007